Correlative Light and Electron Microscopy (CLEM) Techniques for Biological Samples
نویسندگان
چکیده
منابع مشابه
Correlative Light and Electron Microscopy (CLEM) and its applications in infectious disease
Correlative light and electron microscopy (CLEM) is an effective technique used to study biological samples. Signal-specific indicators observable under light microscopy (LM) allow scientists to locate areas of interest for high resolution ultra-structure observation under the electron microscope (EM). Recent method developments provided breakthroughs creating an effective, direct, and accurate...
متن کاملProcessing scarce biological samples for light and transmission electron microscopy.
Light microscopy (LM) and transmission electron microscopy (TEM) aim at understanding the relationship structure-function. With advances in biology, isolation and purification of scarce populations of cells or subcellular structures may not lead to enough biological material, for processing for LM and TEM. A protocol for preparation of scarce biological samples is presented. It is based on pre-...
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Correlative optical microscopy and scanning electron microscopy (SEM) combines two techniques enabling the use both optical and electronic analysis techniques on the same sample. For example, optical techniques, such as fluorescence staining, fluorescence in situ hybridization (FISH) could in principle be used with SEM and energy dispersive spectroscopy (EDS) [1]. Optical microscopy provides ef...
متن کاملCorrelative light and electron microscopy (CLEM) as a tool to visualize microinjected molecules and their eukaryotic sub-cellular targets.
The eukaryotic cell relies on complex, highly regulated, and functionally distinct membrane bound compartments that preserve a biochemical polarity necessary for proper cellular function. Understanding how the enzymes, proteins, and cytoskeletal components govern and maintain this biochemical segregation is therefore of paramount importance. The use of fluorescently tagged molecules to localize...
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ژورنال
عنوان ژورنال: Microscopy and Microanalysis
سال: 2013
ISSN: 1431-9276,1435-8115
DOI: 10.1017/s1431927613002493